NLSdb Help


NLSdb provides multiple services concerning nuclear export and localization signals (NES/NLS).
Users can either submit protein sequences to search for potential NES/NLS, or their own NES/NLS sequences to evaluate or compare them against NLSdb.
We also offer the option to simply browse all NES/NLS in NLSdb, as well as downloads for all NES/NLS sequences and (non-)nuclear proteins.


NLSdb has the following submit options:
Find NES/NLS in protein sequence (FASTA)

Input: One or more protein sequences in FASTA format.
Searches the sequence(s) for any NES/NLS contained in NLSdb.

Find NES/NLS in protein sequence (UniProt AC)

Input: One or more UniProt ACs.
Maps the UniProt AC(s) to the corresponding protein sequence(s) and searches the sequence(s) for any NES/NLS contained in NLSdb.

Find similar NES/NLS in NLSdb

Input: One or more (potential) NES/NLS sequences.
Searches NLSdb for any NES/NLS similar to the query NES/NLS sequence(s).

Evaluate NES/NLS

Input: One or more (potential) NES/NLS sequences.
Evaluates the query NES/NLS based on the number of nuclear and non-nuclear protein sequences it matches (they match).


Query results are provided with (a subset of) the following information:

Query

The original query (FASTA header, UniProt AC, or NES/NLS sequence).

Signal

The NES/NLS sequence matched by the query.

UniProt AC

The UniProtKB AC of the protein sequence matched by the query.

SignalType

The type of the signal (NES or NLS).

ProteinType

The type of the protein (nuclear or non-nuclear).

Start

The start position of the match within the sequence.

End

The end position of the match within the sequence.

ConfNuc

The number of nuclear proteins matched by the query.

ConfFam

The number of nuclear protein families matched by the query.

NonNuc

The number of non-nuclear proteins matched by the query.

AnnotationType

The evidence annotation for the NES/NLS (by expert, experimental, or potential).

Origin

The origin/source of the NES/NLS (in silico mutagenesis, UniProtKB AC, SeqNLS, ValidNESs, or NESbase).

Added

The date the NES/NLS or protein entry was added to NLSdb.

Modifed

The date the NES/NLS or protein entry was last modified (e.g. number of proteins matched).


This is an updated version of NLSdb. The legacy NLSdb can be found here. If you have questions and/or encounter any problems, you can contact us here.
Copyright © 2017 Michael Bernhofer, Tatyana Goldberg and Burkhard Rost, ROSTLAB all rights reserved.

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