Structure-based analysis of CysZ-mediated cellular uptake of sulfate.

TitleStructure-based analysis of CysZ-mediated cellular uptake of sulfate.
Publication TypeJournal Article
Year of Publication2018
AuthorsSanghai, ZAssur, Liu, Q, Clarke, OB, Belcher-Dufrisne, M, Wiriyasermkul, P, M Giese, H, Leal-Pinto, E, Kloss, B, Tabuso, S, Love, J, Punta, M, Banerjee, S, Rajashankar, KR, Rost, B, Logothetis, D, Quick, M, Hendrickson, WA, Mancia, F
JournalElife
Volume7
Date Published2018 May 24
ISSN2050-084X
Abstract

Sulfur, most abundantly found in the environment as sulfate (SO), is an essential element in metabolites required by all living cells, including amino acids, co-factors and vitamins. However, current understanding of the cellular delivery of SO at the molecular level is limited. CysZ has been described as a SO permease, but its sequence family is without known structural precedent. Based on crystallographic structure information, SO binding and flux experiments, we provide insight into the molecular mechanism of CysZ-mediated translocation of SO across membranes. CysZ structures from three different bacterial species display a hitherto unknown fold and have subunits organized with inverted transmembrane topology. CysZ from assembles as a trimer of antiparallel dimers and the CysZ structures from two other species recapitulate dimers from this assembly. Mutational studies highlight the functional relevance of conserved CysZ residues.

DOI10.7554/eLife.27829
Alternate JournalElife
PubMed ID29792261
PubMed Central IDPMC5967866
Grant ListS10 RR029205 / RR / NCRR NIH HHS / United States
P41 GM103403 / GM / NIGMS NIH HHS / United States
P41 GM116799 / / National Institute of General Medical Sciences /
R01 GM098617 / GM / NIGMS NIH HHS / United States
R01 GM107462 / GM / NIGMS NIH HHS / United States
R01 GM107462 / / National Institute of General Medical Sciences /
P41 GM116799 / GM / NIGMS NIH HHS / United States
U54 GM095315 / / National Institute of General Medical Sciences /
R01 GM119396 / / National Institute of General Medical Sciences /
U54 GM095315 / GM / NIGMS NIH HHS / United States
R01 GM098617 / / National Institute of General Medical Sciences /
R01 GM119396 / GM / NIGMS NIH HHS / United States
NYCOMPS; U54 GM095315 / / New York Consortium on Membrane Protein Structure /