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Input sequence
Entry name
Paste, or type in your sequence
  • amino acids in one-letter code
  • any number of white spaces allowed
  • non-standard amino acids to 'U'
  • sequence only (NO ">" or sequence name)


Input substitutions pattern (OPTIONAL) help Substitution pattern format for in silico mutagenesis

Total mutants:

0
Please note, Internet Explorer may have problems generating mutant lists. We are aware of the problem and ask that you bear with us as we are trying to fix it!
  • XposY where X is the wild type amino acid, Y is the mutant, and pos is the number of the residue (pos=1 for N-term)
  • X is NOT required and a star (* = all possible) can replace either pos or Y
  • Press Generate Mutants to see all possible mutations in the text field below
  • Mutations may be added/removed before hitting Submit, but no more than 150 may be submitted per run


Input substitutions help Substitutions format for a list of mutants
  • Paste or type in your substitutions in format XposY, where X and Y are any amino acids and "pos" is the position in sequence (e.g A365N)
  • separate multiple subsitutions by ","
  • any number of white spaces is allowed
  • no more than 150 mutants may be submitted per run


Advanced options
Min reliability index:
help integer between 0 and 9
Min expected accuracy: %
help number between 50 and 100


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